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anti cd31 apc vio 770  (Miltenyi Biotec)


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    Miltenyi Biotec anti cd31 apc vio 770
    Anti Cd31 Apc Vio 770, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd31+apc/CD31+Antibody%2C+anti-human%2C+REAfinity/pmc13058225-44-28-31
    Average 95 stars, based on 79 article reviews
    anti cd31 apc vio 770 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Staining:

    Article Title: Targeted delivery of therapeutics to metastatic lung cancer cells using aptamer-conjugated nanoemulsions.
    Article Snippet: Lung cancer is the primary cause of cancer-related deaths, with a five-year overall survival rate of 15 %, mainly related to late diagnosis at advanced stages.. Despite recent improvements in cancer medicine, metastatic disease still, in fact, represents the main challenge for effective therapies.. Targeting disseminated cells requires a better understanding of the underlying biological mechanisms and a paradigm shift to devise innovative treatments.

    Article Title: Oxidative Stress-Induced Microglial CD22 Upregulation Impairs Phagocytosis and Exacerbates Huntington’s Disease
    Article Snippet: .. The single cell suspension was first blocked with 1:65 Fc block (BD Pharmingen; 553142) for 10 minutes on ice before being stained with 1:10 CD90.2/Thy-1.2–APC/FireTM (BioLegend; 105348), 1:50 ACSA2–PE-Vio ® 770 (Miltenyi Biotec; 130-116-246), 1:200 CD11b–BV421 (BioLegend; 101236), 1:10 anti-Galactocerebroside–FITC (Merck Milli-Mark ® ; FCMAB312F), 1:50 CD31–APC (Miltenyi Biotec; 130-123-813), and 1:1000 Fixable Viability Dye eFluorTM506 (Invitrogen eBioscienceTM; 65-0866-14). ..

    Article Title: Defining the Vascular Niche of Human Adipose Tissue Across Metabolic Conditions
    Article Snippet: .. Isolated cells were spun down, resuspended in 100ul wash buffer, and stained with Hoechst (1:500), CD45-PE-Vio770 (1:50) (130-110-634, Miltenyi Biotec), CD31-APC (1:50) (130-111-354, Miltenyi Biotec), CD34-PE (1:50) (12-0349-41, Invitrogen), and PDGFRA-PE (1:50) (A15785, Invitrogen) for 30 minutes at 4 °C. .. Cells were then washed twice with wash buffer and data were acquired on a NovoCyte 3000 flow cytometer equipped with three lasers (405nm, 488nm, and 640 nm) and 13 fluorescence detectors using the NovoExpress (v. 1.6.2) software (Agilent, Santa Clara, CA).

    Immunopeptidomics:

    Article Title: Targeted delivery of therapeutics to metastatic lung cancer cells using aptamer-conjugated nanoemulsions.
    Article Snippet: Lung cancer is the primary cause of cancer-related deaths, with a five-year overall survival rate of 15 %, mainly related to late diagnosis at advanced stages.. Despite recent improvements in cancer medicine, metastatic disease still, in fact, represents the main challenge for effective therapies.. Targeting disseminated cells requires a better understanding of the underlying biological mechanisms and a paradigm shift to devise innovative treatments.

    Incubation:

    Article Title: Defining the Vascular Niche of Human Adipose Tissue Across Metabolic Conditions
    Article Snippet: One million cells were then stained using Live/Dead fixable blue (L23105, Invitrogen) in 1X PBS (SH30256.01, Cytiva) for 20 minutes at room temperature. .. Subsequently, cells were incubated with an antibody mix (1:200 HAL-DR-BUV615 (G46-6, 751142, BD Optibuild), 1:600 CD206-BUV661 (19.2, 741641, BD Optibuild), 1:300 CD19-BUV805 (HIB19, 742007, BD Optibuild), 1:100 CD127-BV421 (HIL-7R-M21, 562437, BD Biosciences), 1:200 CD11b-BV570 (ICRF44, 301325, Biolegend), 1:200 CD3-BV605 (SK7, 563219, BD Horizon), 1:100 CCR2-BV786 (1D9, 747855, BD Optibuild), 1:50 CDH5-PE (REA199, 130-118-358, Miltenyi Biotec), 1:50 CD31-APC (REA1028, 130-111-354, Miltenyi Biotec), 1:100 CD163-R718 (Mac2-158, 568185, BD Horizon), and 1:1000 CD45-APC-Vio770 (REA747, 130-110-635, Biolegend)) containing human IgG (Privigen) and OligoBlock in FACS buffer (1mM EDTA pH 8.0 (15575-038, Invitrogen), 2.5% fetal calf serum, 0.09% NaN3 (52300.0500, Ampliqon) in 1X PBS pH 7.4) at 4 degrees for 30 minutes. .. Finally, cells were intracellularly stained with 1:50 CD247-PerCP-eFluor710 (6B10.2, 46-247-42, Invitrogen) overnight at 4 degrees using the eBioscienceTM Foxp3/Transcription factor staining buffer set (00-5523-00, Invitrogen) according to the manufacturer’s instructions.

    Article Title: 3D imaging and single-cell analysis reveal cellular heterogeneity of lymphatic valve endothelial cell types
    Article Snippet: Cell suspension was successively filtered through 100μm and 40um cell strainer, and incubated with RBC lysis buffer (eBioscience). .. Cells were then washed with FACS buffer and then incubated with conjugated antibodies CD45 PE-violet770 (130-110-661, Miltenyi) and CD31-APC (130-111-355, Miltenyi). ..

    FACS:

    Article Title: Defining the Vascular Niche of Human Adipose Tissue Across Metabolic Conditions
    Article Snippet: One million cells were then stained using Live/Dead fixable blue (L23105, Invitrogen) in 1X PBS (SH30256.01, Cytiva) for 20 minutes at room temperature. .. Subsequently, cells were incubated with an antibody mix (1:200 HAL-DR-BUV615 (G46-6, 751142, BD Optibuild), 1:600 CD206-BUV661 (19.2, 741641, BD Optibuild), 1:300 CD19-BUV805 (HIB19, 742007, BD Optibuild), 1:100 CD127-BV421 (HIL-7R-M21, 562437, BD Biosciences), 1:200 CD11b-BV570 (ICRF44, 301325, Biolegend), 1:200 CD3-BV605 (SK7, 563219, BD Horizon), 1:100 CCR2-BV786 (1D9, 747855, BD Optibuild), 1:50 CDH5-PE (REA199, 130-118-358, Miltenyi Biotec), 1:50 CD31-APC (REA1028, 130-111-354, Miltenyi Biotec), 1:100 CD163-R718 (Mac2-158, 568185, BD Horizon), and 1:1000 CD45-APC-Vio770 (REA747, 130-110-635, Biolegend)) containing human IgG (Privigen) and OligoBlock in FACS buffer (1mM EDTA pH 8.0 (15575-038, Invitrogen), 2.5% fetal calf serum, 0.09% NaN3 (52300.0500, Ampliqon) in 1X PBS pH 7.4) at 4 degrees for 30 minutes. .. Finally, cells were intracellularly stained with 1:50 CD247-PerCP-eFluor710 (6B10.2, 46-247-42, Invitrogen) overnight at 4 degrees using the eBioscienceTM Foxp3/Transcription factor staining buffer set (00-5523-00, Invitrogen) according to the manufacturer’s instructions.

    Article Title: 3D imaging and single-cell analysis reveal cellular heterogeneity of lymphatic valve endothelial cell types
    Article Snippet: Cell suspension was successively filtered through 100μm and 40um cell strainer, and incubated with RBC lysis buffer (eBioscience). .. Cells were then washed with FACS buffer and then incubated with conjugated antibodies CD45 PE-violet770 (130-110-661, Miltenyi) and CD31-APC (130-111-355, Miltenyi). ..

    Single Cell:

    Article Title: Oxidative Stress-Induced Microglial CD22 Upregulation Impairs Phagocytosis and Exacerbates Huntington’s Disease
    Article Snippet: .. The single cell suspension was first blocked with 1:65 Fc block (BD Pharmingen; 553142) for 10 minutes on ice before being stained with 1:10 CD90.2/Thy-1.2–APC/FireTM (BioLegend; 105348), 1:50 ACSA2–PE-Vio ® 770 (Miltenyi Biotec; 130-116-246), 1:200 CD11b–BV421 (BioLegend; 101236), 1:10 anti-Galactocerebroside–FITC (Merck Milli-Mark ® ; FCMAB312F), 1:50 CD31–APC (Miltenyi Biotec; 130-123-813), and 1:1000 Fixable Viability Dye eFluorTM506 (Invitrogen eBioscienceTM; 65-0866-14). ..

    Suspension:

    Article Title: Oxidative Stress-Induced Microglial CD22 Upregulation Impairs Phagocytosis and Exacerbates Huntington’s Disease
    Article Snippet: .. The single cell suspension was first blocked with 1:65 Fc block (BD Pharmingen; 553142) for 10 minutes on ice before being stained with 1:10 CD90.2/Thy-1.2–APC/FireTM (BioLegend; 105348), 1:50 ACSA2–PE-Vio ® 770 (Miltenyi Biotec; 130-116-246), 1:200 CD11b–BV421 (BioLegend; 101236), 1:10 anti-Galactocerebroside–FITC (Merck Milli-Mark ® ; FCMAB312F), 1:50 CD31–APC (Miltenyi Biotec; 130-123-813), and 1:1000 Fixable Viability Dye eFluorTM506 (Invitrogen eBioscienceTM; 65-0866-14). ..

    Blocking Assay:

    Article Title: Oxidative Stress-Induced Microglial CD22 Upregulation Impairs Phagocytosis and Exacerbates Huntington’s Disease
    Article Snippet: .. The single cell suspension was first blocked with 1:65 Fc block (BD Pharmingen; 553142) for 10 minutes on ice before being stained with 1:10 CD90.2/Thy-1.2–APC/FireTM (BioLegend; 105348), 1:50 ACSA2–PE-Vio ® 770 (Miltenyi Biotec; 130-116-246), 1:200 CD11b–BV421 (BioLegend; 101236), 1:10 anti-Galactocerebroside–FITC (Merck Milli-Mark ® ; FCMAB312F), 1:50 CD31–APC (Miltenyi Biotec; 130-123-813), and 1:1000 Fixable Viability Dye eFluorTM506 (Invitrogen eBioscienceTM; 65-0866-14). ..

    Isolation:

    Article Title: Defining the Vascular Niche of Human Adipose Tissue Across Metabolic Conditions
    Article Snippet: .. Isolated cells were spun down, resuspended in 100ul wash buffer, and stained with Hoechst (1:500), CD45-PE-Vio770 (1:50) (130-110-634, Miltenyi Biotec), CD31-APC (1:50) (130-111-354, Miltenyi Biotec), CD34-PE (1:50) (12-0349-41, Invitrogen), and PDGFRA-PE (1:50) (A15785, Invitrogen) for 30 minutes at 4 °C. .. Cells were then washed twice with wash buffer and data were acquired on a NovoCyte 3000 flow cytometer equipped with three lasers (405nm, 488nm, and 640 nm) and 13 fluorescence detectors using the NovoExpress (v. 1.6.2) software (Agilent, Santa Clara, CA).

    other:

    Article Title: 3D imaging and single-cell analysis reveal cellular heterogeneity of lymphatic valve endothelial cell types
    Article Snippet: CD31-APC , Miltenyi , Cat#130-111-355; RRID: AB_2657299.



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